UJI TINGKAT KEKONTRASAN PREPARAT JARINGAN OTOT MENGGUNAKAN PEWARNA ALAMI DARI LARUTAN UMBI BIT (Beta vulgaris L) SEBAGAI SUMBER BELAJAR BIOLOGI

Abstract

Cell or tissue dyes that have been used are synthetic dyes where the
chemicals are relatively expensive and carcinogenic in nature. Alternative
dyes can be used are natural dyes that have a high affinity for the components
of cells or tissues. The aim of this study was to determine the contrast level of
muscle tissue preparations stained using solution (Beta vulgaris L) and
determine the concentration of solution (Beta vulgaris L) which had the best
effect on the level of muscle tissue contrast.
This type of research is an experimental study with qualitative
descriptive data processing, the design uses 6 treatment variations of beetroot
concentration concentration (20%, 50%, 60%, 75%, 90%, 100%) and 2
treatments as a control (without coloring and eosin coloring) with 3
replications. Data obtained by direct observation using a microscope and
documented with a camera and then conducted a review by biologists. The
parameter observed was the contrast of the color of the muscle tissue
preparation. The results showed that the contrast level of (Columna livia)
skeletal muscle tissue preparations stained using solution (Beta vulgaris L) was
best at a concentration of 60% was very clear criteria and very contrasted with
the average value obtained 2, 74. The results of this study were compiled as a
source of learning biology in the form of practical instructions for class XI high
school students in the structure and function of tissue in animals with an
average percentage worth of 86%.